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General course topics

Course: BSO-BSL1

The players in the Swiss biosafety law and their responsibilities - Resources for information - Goals of biosafety - Transmission pathways - Laboratory acquired infections (LAI) / aerosols - (Background on) Risk groups with examples - Risk assessment - Safety levels - Case studies - Safe working, hand hygiene - Personal protective equipment (PPE) - Laboratory instruments - Biosafety cabinets - Inactivation procedures - Chemical disinfection - Transport - Safety concept - Spill

Course: BSO-BSL2

Basics in biosafety - Waste Management - Safe working with biorisks - Biological hazards and LAI, Containment principles - Laboratory design and technical equipment - Laboratory- and safety equipment - Personal protective equipment - Risk assessment and risk management - Packing, sending, transport, import and export of biological agents - Hygiene, disinfection, decontamination, sterilisation - Biosafety training - Discussion, Questions, Evaluation

Course BSO-BSL3

Definition of RG 3 - Risk groups 2, 3** and 3 - Safety levels - Risk assessment - Animal biosafety issues: Hazards associated with animal work - Housing and caging systems - PPE: Basics - Differences to level 2 - Respiratory protection - Hand protection - Body protection - Mask fit test - PPE exercise - Safety equipment: Biosafety cabinets - Centrifuges - Other safety relevant equipment - Test and validation of biosafety cabinet - Barrier principles: Primary barrier- Secondary barrier- Tertiary barrier - Architecture – Facility design – Maintenance and repair: Building - HVAC - Air locks, showers - Material and personal flow - Waste water treatment systems - Design of a level 3 lab - Biorisk management – Training – Internal audits – Safety culture - Inspections – Ordinance on major accidents - Decontamination, inactivation and waste management: Chemical, physical methods - Fumigation systems for laboratories and BSC - Validation of inactivation - Waste disposal systems - Emergency procedures – Contingency planning - Emergencey Exercise - Biosecurity - Evaluation

Advanced subject-specific training: Biosecurity

The history of biological weapons and the inherent dual use problem in the life sciences – Practical exercise and examples of dual use research and ‘dual use research of concern ‘ (DURC), how various countries address the dual use problem, and practical approaches to adequately address the dual use problem – Approaches to laboratory biosecurity with practical examples – The potential role of biosafety officers in terms of biosecurity - Questions and discussion

Advanced subject-specific training: Autoclaves and waste disposal

Legal basis on waste (waste directive of the FOEN, directives of the SECB) - Sterilisation / disinfection (basics, requirements, possibilities and limitation, tests, MSDS, personal protective equipment) - Function of autoclaves (gravitation / vakuum, solid and liquid waste, exhaust air filters) - Testing (chemical, biological, electronic) - Installation and maintenance - Questions and discussion

Advanced subject-specific training: Risk_Vectors

Day 1

Risk assessment of bacteria, viruses, parasites, fungi, plants and animals and risk groups of organisms - Risk assessment of genetic modifications and classification of activities - Discussion of case studies with actual examples that were handed in to the federal authorities including how the authorities deal with these - Influence of the risk assessment to risk management.

Day 2 (morning)

General overview of viral vectors and criteria for risk assessment - AAV- and adenoviral vectors - Lentiviral vectors - Rabies and pseudorabies vectors as well as questions of the participants of vectors that have not been discussed.

These vectors were chosen according to the frequency they are used in Swiss research laboratories.

Advanced subject-specific training: Inactivation of liquids

Legal base - Advantages / disadvantages in comparison to autoclaving - Chemical substances: approval, host range, environmental impact, worker safety - Definitions (D-value, inactivation, disinfection etc.) -  Development of a new chemical disinfaction process - Shelf life of a disinfectant - Variation of parameters such as duration of exposure, concentration of disinfectant etc. - Inactivation in "worst case“ scenarios - Evaluation of the process - Chemical, physical indicators and / or biological indicators - Testing of efficacy, necessary controls - Questions and discussion

© Curriculum Biosafety, April 19, 2022